anti cd3 fitc Search Results


95
Elabscience Biotechnology staining with anti cd3 fitc
Staining With Anti Cd3 Fitc, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/pmc11658117-150-6-13?v=Elabscience+Biotechnology
Average 95 stars, based on 1 article reviews
staining with anti cd3 fitc - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier

93
Cedarlane anti rat cd3
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Anti Rat Cd3, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/pmc08116535-147-27-34?v=Cedarlane
Average 93 stars, based on 1 article reviews
anti rat cd3 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

93
Elabscience Biotechnology anti human cd3 apc cd4fitc cd8a pe cocktail
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Anti Human Cd3 Apc Cd4fitc Cd8a Pe Cocktail, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/10__1016_slash_j__jff__2025__106816-72-10-33?v=Elabscience+Biotechnology
Average 93 stars, based on 1 article reviews
anti human cd3 apc cd4fitc cd8a pe cocktail - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

94
Elabscience Biotechnology anti human cd3
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Anti Human Cd3, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/pm39753573-301-11-13?v=Elabscience+Biotechnology
Average 94 stars, based on 1 article reviews
anti human cd3 - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier

93
Proteintech proteintech cat
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Proteintech Cat, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/pm41832952-458-47-47?v=Proteintech
Average 93 stars, based on 1 article reviews
proteintech cat - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

93
Cytek Biosciences anti human cd3
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Anti Human Cd3, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/pm30924167-74-28-32?v=Cytek+Biosciences
Average 93 stars, based on 1 article reviews
anti human cd3 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

91
Diaclone anti human cd3
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Anti Human Cd3, supplied by Diaclone, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/pmc00421644-190-33-37?v=Diaclone
Average 91 stars, based on 1 article reviews
anti human cd3 - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier

91
Proteintech cd3 fitc
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Cd3 Fitc, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/pmc10132258__SC___014___D2SC06036A___s001-34-6-15?v=Proteintech
Average 91 stars, based on 1 article reviews
cd3 fitc - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier

93
Elabscience Biotechnology cd56 pe
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Cd56 Pe, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/bio_rxiv__2025__08__20__671208-233-20-22?v=Elabscience+Biotechnology
Average 93 stars, based on 1 article reviews
cd56 pe - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

93
Cytek Biosciences anti cd3 fitc
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Anti Cd3 Fitc, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/pm39168069-60-19-22?v=Cytek+Biosciences
Average 93 stars, based on 1 article reviews
anti cd3 fitc - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

93
Elabscience Biotechnology anti human cd3 fitc cd19 apc cd16 cd56 pe cocktail
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Anti Human Cd3 Fitc Cd19 Apc Cd16 Cd56 Pe Cocktail, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/10__1016_slash_j__jff__2025__106816-72-24-33?v=Elabscience+Biotechnology
Average 93 stars, based on 1 article reviews
anti human cd3 fitc cd19 apc cd16 cd56 pe cocktail - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

94
Cytek Biosciences anti cd3 mab
Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and <t>CD3</t> + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.
Anti Cd3 Mab, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3+fitc/us11806393-629-9-13?v=Cytek+Biosciences
Average 94 stars, based on 1 article reviews
anti cd3 mab - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier

Image Search Results


Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and CD3 + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.

Journal: Frontiers in Immunology

Article Title: Desmoglein-4 Deficiency Exacerbates Psoriasiform Dermatitis in Rats While Psoriasis Patients Displayed a Decreased Gene Expression of DSG4

doi: 10.3389/fimmu.2021.625617

Figure Lengend Snippet: Dsg-4 deficiency induces high mRNA expression of inflammatory genes in response to IMQ. RNA from skin was extracted, and subsequently real-time PCR and cDNA synthesis were performed to evaluate mRNA abundance of cytokines: IL-1β (A) , IL-8 (B) , IL-17 (C) , IL-10 (D) , and TGF-β (E) performing Kruskal–Wallis tests analysis. Additionally, TGF-β/IL-17 mRNA abundance means ratio was calculated (F) . Similarly, chemokine receptors mRNA levels were measured: CCR1 (G) , CCR2 (H) , CCR3 (I) , CCR5 (J) , and CXCR5 (K) . Kruskal–Wallis and ANOVA tests were used; data represent the median with values range; * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets. (L) Pseudocolor dot plots represent counts of infiltrating CD45 + cells and CD3 + cells from total skin cells analyzed by flow cytometry derived from SD and OFA rats. (M,N) Graphs represent the percentage and absolute cell counts of CD45 + CD3 + cells from total skin cells. ( n rats /group = 6; two independent experiments). (O,P) Spleen and inguinal lymph node weights. FACS and lymphatic tissue weight data are represented as the mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 with ANOVA and Bonferroni post hoc test was used. # P < 0.05, ## P < 0.01 t -test between SD and IMQ-SD datasets.

Article Snippet: The cells were then resuspended in PBS/2% FBS and stained with PE-Cy5-conjugated anti-rat CD45 (clone OX-1; isotype mouse IgG1 k, BD Biosciences, San Jose, USA) and FITC-conjugated anti-rat CD3 (clone IF4; isotype mouse IgM, Cedarlane Laboratories, US).

Techniques: Expressing, Real-time Polymerase Chain Reaction, cDNA Synthesis, Flow Cytometry, Derivative Assay